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HPLC Columns

Target -NH2

● the unique innovative design of the bonding stationary phase is adopted. The silica gel with high purity and good mechanical stability is used as the matrix. By using high purity bonding reagent, the amino group coverage can be realized to the maximum extent, and the carbon content can be as high as 4.0%.
● by strictly controlling the chemical reaction conditions for the formation of polymerized monolayer, reliable reproducibility between columns can be ensured. Target-nh2 surface coverage has been maximized, so it has excellent stability.
The filler is a uniform spherical particle with a pore diameter of 110 μ m and a specific surface area of 310m2 / g. The column bed density of target-nh2 obtained by using the unique homogenate loading technology is uniform and stable, so it can ensure the highest column efficiency.
The target-nh2 column is particularly suitable for the separation of polar and hydrophilic compounds with high selectivity. Because the binding phase of target-nh2 column is 3-aminopropyl, the retention of most nonpolar compounds will be very small.
● target-nh2 column has good separation effect in organic solvent or mixture of water and organic solvent (such as methanol, acetonitrile, etc.). This column is especially suitable for the separation of polar and nonpolar compounds such as drugs, polysaccharides, amino acids, oligonucleotides, oligosaccharides and organic acids.
● stability and performance the target-nh2 column uses a fully covered bonded silica gel filler, so it has excellent chemical stability, especially suitable for the confirmation of many analytes. The chemical bonding technology adopted can avoid the formation of a compound aminopropyl molecular layer. The stationary phase with this uniform coating has the characteristics of high selectivity and high separation efficiency. Separation may be carried out in a non-polar solvent such as hexane or in a polar solvent such as an aqueous solution of acetonitrile.
● pH range pH 2-8.5
● It can be used in two separation modes, positive phase and reverse phase
● A variety of mobile phase systems are available, such as hexane / ethyl acetate, chloroform / methanol, aqueous solution, etc
● It can be used for supercritical fluid chromatography (SFC) separation and LC / MS analysis
Chromatographic conditions of sucrose, fructose and glucose:
Column:Target -NH2( 5µm, 4.6 x 250 mm )
Mobile phase:H2O : ACN= 25 : 75 ( v/v )
Flow rate:1.0mL/min
Temperature:35℃
Detection:ELSD ( 60℃,2 mL/min )
Injection volume:5µL
Sample:Sucrose ( 1.28mg/mL ), fructose ( 1.37mg/mL ) and glucose ( 0.52mg/mL )

Maltose chromatography conditions:
Column:Target -NH2( 5 µm, 4.6 x 250 mm )
Mobile phase:ACN : H2O = 70 : 30 ( v/v )
Flow rate:1.0 mL/min
Temperature:35 ℃
Detection:RI 30℃
Injection volume:20 µL
Sample:sample ( 10.0 mg/mL )

Target -NH2 Product specifications

name

Particle size

Specifications

Part No

Target -NH2

3μm

2.1mm×30mm

310003-2103

Target -NH2

3μm

2.1mm×50mm

310003-2105

Target -NH2

3μm

2.1mm×100mm

310003-2110

Target -NH2

3μm

2.1mm×150mm

310003-2115

Target -NH2

3μm

2.1mm×250mm

310003-2125

Target -NH2

3μm

3.0mm×30mm

310003-3003

Target -NH2

3μm

3.0mm×50mm

310003-3005

Target -NH2

3μm

3.0mm×100mm

310003-3010

Target -NH2

3μm

3.0mm×150mm

310003-3015

Target -NH2

3μm

3.0mm×250mm

310003-3025

Target -NH2

3μm

4.6mm×30mm

310003-4603

Target -NH2

3μm

4.6mm×50mm

310003-4605

Target -NH2

3μm

4.6mm×100mm

310003-4610

Target -NH2

3μm

4.6mm×150mm

310003-4615

Target -NH2

3μm

4.6mm×250mm

310003-4625

Target -NH2

5μm

2.1mm×30mm

310005-2103

Target -NH2

5μm

2.1mm×50mm

310005-2105

 

 

 

 

Target -NH2

5μm

2.1mm×100mm

310005-2110

Target -NH2

5μm

3.0mm×30mm

310005-3003

Target -NH2

5μm

3.0mm×50mm

310005-3005

Target -NH2

5μm

3.0mm×100mm

310005-3010

Target -NH2

5μm

3.0mm×150mm

310005-3015

Target -NH2

5μm

3.0mm×250mm

310005-3025

Target -NH2

5μm

4.0mm×250mm

310005-4025

Target -NH2

5μm

4.0mm×300mm

310005-4030

Target -NH2

5μm

4.6mm×30mm

310005-4603

Target -NH2

5μm

4.6mm×50mm

310005-4605

Target -NH2

5μm

4.6mm×100mm

310005-4610

Target -NH2

5μm

4.6mm×150mm

310005-4615

Target -NH2

5μm

4.6mm×200mm

310005-4620

Target -NH2

5μm

4.6mm×250mm

310005-4625

Target -NH2

5μm

4.6mm×300mm

310005-4630

Target -NH2

5μm

10.0mm×50mm

310005-10005

Target -NH2

5μm

10.0mm×100mm

310005-10010

Target -NH2

5μm

21.2mm×50mm

310005-21205

Target -NH2

5μm

21.2mm×100mm

310005-21210

Target -NH2

5μm

30.0mm×50mm

310005-30005

Target -NH2

5μm

30.0mm×100mm

310005-30010

Target -NH2

5μm

50.0mm×50mm

310005-50005

Target -NH2

5μm

50.0mm×250mm

310005-50025

Target -NH2

10μm

4.6mm×150mm

310009-4615

Target -NH2

10μm

4.6mm×250mm

311009-4625

Target -NH2

10μm

7.8mm×150mm

311009-7815

Target -NH2

10μm

7.8mm×250mm

311009-7825

Target -NH2

10μm

10.0mm×50mm

311009-10005

Target -NH2

10μm

10.0mm×100mm

311009-10010

Target -NH2

10μm

10.0mm×150mm

311009-10015

Target -NH2

10μm

10.0mm×250mm

311009-10025

Target -NH2

10μm

21.2mm×50mm

311009-21205

Target -NH2

10μm

21.2mm×100mm

311009-21210

Target -NH2

10μm

21.2mm×150mm

311009-21215

Target -NH2

10μm

21.2mm×250mm

311009-21225

Target -NH2

10μm

30.0mm×50mm

311009-30005

Target -NH2

10μm

30.0mm×100mm

311009-30010

Target -NH2

10μm

30.0mm×150mm

311009-30015

Target -NH2

10μm

30.0mm×250mm

311009-30025

Target -NH2

10μm

50.0mm×50mm

311009-50005

Target -NH2

10μm

50.0mm×250mm

311009-50025


   * If you need other specifications that are not in this list, please contact your sales representative.

Before:Target-SAX(II)

Before:Target-Silica

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