Target organic acid and sugar alcohol column
Target hydrogen uses sulfonated crosslinked styrene divinylcopolymer hydrogen type cation exchange resin as filler, the crosslinking degree of filler is 5%, 8%, 10%, and the particle size is 5 μ m and 10 μ M.
Target - hydrogen column is specially designed for the efficient separation of sugars, alcohols and organic acids. The new packing was obtained by modifying sulfonic acid group (- SO3H) on the surface of PS / DVB particles with low crosslinking degree. The narrow particle size distribution ensures a high separation rate, while the lower crosslinking degree ensures that it can swell in the mobile phase, thus having a suitable specific surface area and column capacity.
Separation mechanism:
The separation mechanism of target hydroge stationary phase includes ion exchange and hydrophilic interaction with the analyte, such as sugar and organic acid. In addition, the mechanism of volume exclusion, ion exclusion and ligand exchange are also involved. A variety of interaction modes make the target hydrogen column have the unique ability to separate a variety of water-soluble compounds. The crosslinking degree of resin is an important parameter in separation. PS/DVB resin is a relatively rigid gel medium. The lower the degree of crosslinking, the easier the pore structure swelling, and the more favorable the permeability of macromolecular compounds. Therefore, target hydroge resin with 5% cross-linking degree is more suitable for the separation of high molecular weight oligosaccharides than that with 10% cross-linking degree. The compounds with small molecular weight can be separated by target hydroge resin with 8% cross-linking degree.
Column parameters:
We can provide a variety of target hydroge columns with inner diameter ranging from 75 μ m to 21.2 mm and length ranging from 5 cm to 30 cm. In addition, the chromatographic column can be customized according to the requirements of customers. The length and inner diameter of the column will affect the separation effect and the time required for analysis. Therefore, when selecting the specifications of target hydrogen column, it is necessary to ensure that the resin quantity in the selected column can achieve the required separation effect. If the retention of the compound on the resin is too strong, for example, if the retention time on 7.8 300 mm column is too long, a short column such as 150 mm column can be selected to shorten the analysis time
In the separation process under the chromatographic conditions of Pharmacopoeia, the symmetry of ribavirin peak is good, and the number of theoretical plates reaches 8823, which is far higher than the requirement of "the number of theoretical plates calculated according to ribavirin peak is not less than 2000" stipulated in Pharmacopoeia.
Chromatographic conditions of ribavirin
Column: target - hydrogen, 5%, 10 μ m, 7.8 x 300mm
Mobile phase: water (adjusted to pH 2.5 with sulfuric acid)
Flow rate: 0.6ml/min
Detection: UV 207 NM
Column temperature: 55 ℃ injection volume: 20 μ l pressure: 420 PSI
Sample: ribavirin (50 μ g / ml)
| name | retention time | %Area | Tailing factor | Number of trays |
| ribavirin | 20.96 | 100.00 | 1.014 | 8823 |
|
model |
Particle size |
Crosslinking degree |
Specifications |
Article No |
|
Target- Hydrogen |
5μm |
8% |
10.0mm×300mm |
311608-10030 |
|
Target- Hydrogen |
10μm |
5% |
4.6mm×300mm |
311605-4630 |
|
Target- Hydrogen |
10μm |
5% |
7.8mm×100mm |
311605-7810 |
|
Target- Hydrogen |
10μm |
8% |
4.6mm×30mm |
311608-4603 |
|
Target- Hydrogen |
10μm |
8% |
4.6mm×300mm |
311608-4630 |
|
Target- Hydrogen |
10μm |
10% |
7.8mm×300mm |
311610-7830 |
|
Target- Hydrogen |
5μm |
8% |
4.6mm×50mm |
311608-4605 |
|
Target- Hydrogen |
5μm |
8% |
7.8mm×50mm |
311608-7805 |
|
Target- Hydrogen |
5μm |
8% |
4.6mm×250mm |
311608-4625 |
|
Target- Hydrogen |
5μm |
8% |
7.8mm×150mm |
311608-7815 |
|
Target- Hydrogen |
10μm |
8% |
7.8mm×300mm |
311608-7830
|
|
Target- Hydrogen |
10μm |
8% |
10.0mm×300mm |
311608-10030 |
|
Target- Hydrogen |
10μm |
8% |
21.2mm×300mm |
311608-21230 |
