Target ghost column

Detailed introduction of target ghost
Description of ghost peak: in the process of liquid chromatography separation, especially in gradient elution, it is easy to produce unexplained peaks, which are commonly known as ghost peak. Ghost peak comes from many sources, including mobile phase and pipeline, such as: pollutants in organic phase, pollutants in water phase, buffer salt, mobile phase bottle and mixing process.
Once ghost peak appears in the development of methods, it will take more time and energy for analysts to eliminate it, which is also a very difficult work. Now, the problem is finally solved!
Ghost peak capture can effectively capture the ghost peak generated in the process of mobile phase, pipeline and mixing, eliminate the interference of ghost peak on method validation and trace and trace substance analysis, and greatly shorten the time of research and development personnel.
Capture effect beyond imagination: continuous capture of impurities, organic solvents are also applicable
The main feature of ghost peak trapping column is that it can remove impurities in solvents including organic solvents. When the column is installed between the gradient mixer and the autosampler, not only the impurities in the mobile phase can be removed, but also the impurities in the pipeline and mixer can be effectively trapped. As shown below:

Source of ghost peak:
There are many ways for water to bring impurities to the purification system itself. Storage containers are introduced and placed for too long, leading to the growth of bacteria. Various mobile phase additives, salts, acids, alkalis and other organic pollutants are no longer pure for a long time.
Hazards of Guifeng:
1. Contaminated instrument
2. Misjudgment of sample composition
3. Overlapping with the target peak, affecting the resolution
4. More work needs to be done to verify whether the substance is the target substance
working principle:
There are many on-line filtration devices on the market, mainly for the interception of solid particles, but they can not intercept the organic pollutants causing ghost peak. Target ghost small column has strong adsorption on weak polar and non-polar organic impurities, but does not change the packing of mobile phase composition (without ion pair reagent). It carries out secondary purification on the instrument and mobile phase, effectively eliminates such pollutants, reduces the probability of ghost peak occurrence, and prolongs the life of chromatographic column and instrument.
Installation and use:
1. When using, install the impurity trapping column between the gradient mixer and the injector. Make sure that the column is installed before the injector. If it is installed after the injector, it will have strong adsorption on the sample.
2. Please use 80% methanol water to wash the target ghost impurity collection column for 1ml / min, flow rate for 20min, and then connect it to the equipment for use
3. Not all impurities can be absorbed by target ghost.
4. If the ion pair reagent is used in the mobile phase, it may adsorb the ion pair reagent and affect the retention time or peak type of the target substance. For this kind of mobile phase conditions, please determine whether to use the small column according to the chromatographic effect.
5. The life of chromatographic column is related to chromatographic analysis conditions, mobile phase brand and its purity. If the trapping effect becomes poor, it is recommended to replace it in time, but it can not be used indefinitely after being connected.
6. As a purification widget of the instrument, target ghost is equivalent to on-line filter on the instrument, only on-line filter can only filter solid particles, and it can not only filter solid particles, but also purify organic pollutants in the system, which is a better protection for the instrument and chromatographic column.
7. Before and after the use of buffer salt mobile phase, pay attention to use high proportion of water for transitional flushing to avoid buffer salt out, resulting in packing blockage.
8. When it is found that the collection effect of the small column is not ideal, it can be tried to wash the small column with 100% acetonitrile, and the outlet end of the small column should not be connected to the instrument.
Effect comparison:

Explanation: A is the injection map; B is the effect after adding the target ghost ghost small column.
It can be seen that the target ghost impurity trap column can effectively remove the unknown impurities in the mobile phase solvent.
Ordering information:

name	Specifications	Article No	Withstand pressure	Application equipment
Target-Ghost	2.1*50mm	31805-2105	1000bar	LCMS
Target-Ghost	4.6*75mm	31805-4607	600bar	HPLC