Target ephedrine special column
Time：2020-05-22 10:23Click：57
The special column for ephedra was filled with polar ether and phenyl bonded silica gel, and the packing was uniform spherical particle with carbon loading of 11.0%. In the separation process under Pharmacopoeia chromatographic conditions, the peak symmetry of ephedrine and pseudoephedrine is good, and the number of theoretical plates is more than 10000, which is far higher than the requirement of "the number of theoretical plates should not be less than 3000 calculated according to the peak of ephedrine hydrochloride" stipulated in Pharmacopoeia.Chromatographic conditions of ephedrine hydrochloride
Chromatographic column: target - ephedrine (5 μ m, 4.6 × 250 mm)
Mobile phase: 0.092% phosphoric acid solution (containing 0.04% triethylamine and 0.02% di-n-butylamine-methanol (98.5:1.5) (V / V)
Flow rate: 1.0ml/min
Column temperature: RT
Detection: UV 210 nmSample: 1. Ephedrine hydrochloride (0.03768 mg / ml)
2. Pseudoephedrine hydrochloride (0.04648 mg / ml)
Injection volume: 10 μ l pressure: 2100 PSI
| name | retention time | %Area | Resolution | Tailing factor | Number of trays |
| unknown | 3.75 | 4.04 | 1.79 | 3837.7 | |
| ephedrine | 9.39 | 43.16 | 18.52 | 0.93 | 10649.1 |
| Pseudoephedrine | 11.56 | 52.79 | 5.18 | 1.29 | 10144.2 |
| Article No | Product description | Sample test |
| 301803-4625 | Target -Ephedrine 5um 250*4.6mm | Special column for Ephedra |
