Target-NH2 (II), a special column for lactose, is obtained by bonding amino groups on silica gel with high purity bonding reagent. In the separation process under Pharmacopoeia chromatographic conditions, the detection of lactose and sucrose, the resolution completely meets the requirements of Pharmacopoeia, the theoretical plate number calculated by lactose peak is more than 8000, far higher than the requirements of Pharmacopoeia.

 

Lactose chromatographic conditions

 

Chromatographic column: target-nh2 (II), 5 μ m, 4.6 × 300 mm

 

Mobile phase: ACN: H2O = 70: 30 (V / V)

 

Flow rate: 1.0ml/min

 

Column temperature: 45oC

 

Test: differential (40 ℃)

 

Sample: system suitability solution (1.0 mg / ml lactose + 1.0mg / ml sucrose)

 

Injection volume: 10 μ L

name	retention time	the measure of area	Tailing factor	Number of trays	Resolution
sucrose	10.93	84432	1.26	12213
lactose	13.21	49633	1.18	9011	4.77

Part No	Product description	Sample test
301807-4630	Target-NH2(II) 5um  300x4.6mm	Lactose column