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HPLC Columns

Target-Insulin

Target insulin special column
TheTarget-Insulin  binding phase of insulin specific column is prepared by bonding a uniform nano thickness hydrophilic layer on a silica gel matrix with high purity and good mechanical stability, using a patented surface modification technology. In the separation process under Pharmacopoeia chromatographic conditions, the detection of insulin high molecular protein showed that the resolution of islet dimer and monomer completely met the requirements of Pharmacopoeia.
Chromatographic conditions of insulin
Chromatographic column: target insulin, 3μm, 80 μm, 7.8 × 300 mm
Mobile phase: acetic acid: acetonitrile: 0.1% arginine solution = 15:20: 65 (V / V)
Flow rate: 0.5ml/min
Column temperature: 40℃
Detection: UV 276nm
Sample: 4mg/ml insulin dissolved in 0.01M HCl solution
Injection volume: 100μL

name

retention time 

the measure of area

Tailing factor

Number of trays

Resolution

Pancreatin dimer

12.745 

1311 

-0.91 

3461 

  

Insulin monomer

14.909 

38217 

0.68 

4123 

2.41 

Sample name                           Specification and model                 Part No
Insulin specific column          ME-Insulin 5u 7.8mm x 300mm      511328-7830
* If you need other specifications that are not in this list, please contact your sales representative.

Before:Target-CN

Before:Target--HILIC

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