MS-Clean Aflatoxin M1
Summary:Aflatoxin M1 is a kind of aflatoxin. After intake of feed or food contaminated by aflatoxin B1, mammalian animals are transformed into aflatoxin M1 by hydroxylation. The harm of aflatoxin M1 is mainly manifested in carcinogenicity and mutagenicity, which can damage human and animal liver tissues and even lead to liver cancer and death. Because aflatoxin M1 poses a great threat to human health, there are strict limit standards for its existence in milk and dairy products all over the world.
Measuring principle:
The immunoaffinity column is based on the antibody antigen specific reaction and contains a gel combined with aflatoxin M1 antibody. When the sample is loaded, the extracted, filtered and diluted sample slowly passes through the immunoaffinity chromatography column of aflatoxin M1. In the column, aflatoxin M1 combines with antibody, and other substances will not combine and flow out directly. After that, the immunoaffinity column was washed and other unbound substances were completely removed. ***After that, the mixture of methanol and acetonitrile was added, aflatoxin M1 was released and eluted, and then injected into the analyzer for detection.
Application scope and purpose:
Aflatoxin M1 immunoaffinity column is suitable for purification of aflatoxin M1 in samples with complex matrix and low limit requirements. It can be used for TLC, HPLC, GC, LC-MS / MS, EIA and other quantitative analysis. It is suitable for milk and dairy products.
Determination conditions of liquid chromatography:
Mobile phase: acetonitrile: water = 25:75
Flow rate: 1 mL/min
Injection volume: 20-500 UL
Column: C18, 4.6x250mm
Fluorescence detection wavelength: emission wavelength 360nm; excitation wavelength 430nm
Inject the standard working solution of aflatoxin M1 into the high performance liquid chromatograph with the injector, and measure the response value (peak height or peak area) of the standard solution under the above chromatographic conditions to obtain the high performance liquid chromatogram of the standard solution of aflatoxin M1.
Product parameters and specifications:
|
Article No |
MSAFMM101 |
MSAFMM103 |
brand |
|
Specifications |
25 pieces / box |
20 pieces / box |
MS-clean |
|
Column volume |
1ML |
3ML |
|
|
Column capacity |
100ngAflatoxin M1 |
150ngAflatoxin M1 |
|
|
|
2-8℃ |
2-8℃ |
