The maintenance of chromatographic column is very important

HPLC (high performance liquid chromatography) is an important means of "separation" in the laboratory, and the chromatographic column can be called the heart of separation. The daily use of HPLC will inevitably encounter various failures caused by chromatographic column, so it is very important to do a good job in the maintenance of chromatographic column in advance. Today, Xiaobian will send you a set of small secrets for the maintenance of chromatographic column, referred to as "Weimi".
1、 The test sample should be filtered
If the insoluble matter in the test sample enters the chromatographic column, it will cause the inlet filter and packing to be blocked, and the column pressure will rise, which will destroy the separation. Therefore, the sample should be injected as clean as possible. In any case, it is recommended to filter the sample with a filter of about 0.5 μ M.
2、 Beware of column contamination
Sometimes, the chromatographic column will be blocked and polluted after the sample is filtered, which may be caused by the impurities in the sample to separate the poor solubility components to the mobile phase, the adsorption of the inclusion components firmly maintained by the packing, and the pollution of the micro syringe. Therefore, it is better to use a filter and protective column before analysis to remove the pollution as much as possible. The filter is usually equipped with a filter screen with an aperture of about 1 μ m, which can effectively remove the substances causing blockage; the protective column is a short column installed in front of the analysis column, which plays a role in capturing the impurities firmly adsorbed on the filler.
3、 Keep the chromatographic column clean
The protective column mentioned above has a limited effect on removing pollution. Therefore, the chromatographic column should be cleaned once after each sample is analyzed according to different samples.
4、 Handle the column with care
The chromatographic column is filled with tiny particles of several microns in size, so the impact and sharp change of external pressure should be avoided to avoid damaging the column.
5、 Confirm the restriction of mobile phase
Sometimes the chromatographic column will be restricted by the pH of mobile phase and the amount of organic solvent, so it needs to be fully confirmed before use.
6、 Use different column responsibilities as required
If a column is used for various analyses, sometimes the previous test data will not be reproduced. In addition, repeated analysis with different mobile phase conditions will accelerate the degradation of chromatographic column. Therefore, it is necessary to record the use of each column.
For more related technologies, please consult MS technologies technicians