Target carbohydrate analysis column
(packing resin and structure diagram of target carbohydrate analysis chromatography)
Target carbohydrate series analytical column is composed of polystyrene / (PS / DVB) as the matrix, cross linked with various ions. Taget There are three crosslinking degrees (5%, 8%, 10%) and five kinds of ions, such as H +, Ca2 +, Pb2 +, K +, Na +, are found in the packing of carbohydrate series analytical column; It is widely used in the separation and preparation of organic acids and carbohydrates, polypeptides and small biomolecules in cell metabolites.
Characteristics of target carbohydrate analysis column
1) The uniform packing particle size ensures high column efficiency
2) Low column pressure and high resolution
3) High temperature resistance 85 ℃
4) High chemical stability and long column life
Related technical parameters of target carbohydrate analysis chromatographic column packing
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Column model |
Target-Hydrogen |
Target-Calcium |
Target-Pb |
Target-K |
Target-Na |
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Filler matrix |
PS/DVB |
PS/DVB |
PS/DVB |
PS/DVB |
PS/DVB |
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Particle size (μm) |
5, 6, 8, 9, 10 |
5, 6, 8, 9, 10 |
5, 6, 8, 9, 10 |
5, 6, 8, 9, 10 |
5, 6, 8, 9, 10 |
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Crosslinking degree |
5%, 8%、10% |
5%, 8%、10% |
5%, 8%、10% |
5%, 8%、10% |
5%, 8%、10% |
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Filler ion type |
H+ |
Ca2+ |
Pb2+ |
K+ |
Na+ |
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ph stability |
1–3 |
5–9 |
5–9 |
5–9 |
5–9 |
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Maximum withstand voltage |
10% crosslinking degree |
10 μm |
1,500 psi |
1,500 psi |
1,500 psi |
1,500 psi |
1,500 psi |
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|
|
5 μm |
1,000 psi |
1,000 psi |
1,000 psi |
1,000 psi |
1,000 psi |
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|
8% crosslinking degree |
10 μm |
1,000 psi |
1,000 psi |
1,000 psi |
1,000 psi |
1,000 psi |
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|
|
5 μm |
800 psi |
800 psi |
800 psi |
800 psi |
800 psi |
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|
5% crosslinking degree |
10 μm |
600 psi |
600 psi |
600 psi |
600 psi |
600 psi |
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|
|
5 μm |
400 psi |
400 psi |
400 psi |
400 psi |
400 psi |
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mobile phase |
PH 2.5 acidic aqueous solution(H2SO4、H3PO4、TFA…) |
Ultra pure water |
Ultra pure water |
Ultra pure water |
Ultra pure water |
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Recommended flow rate(mL/min) |
7.8x300 mm column |
0.4-0.8 |
0.4-0.8 |
0.4-0.8 |
0.4-0.8 |
0.4-0.8 |
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|
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4.6x300 mm column |
0.1-0.3 |
0.1-0.3 |
0.1-0.3 |
0.1-0.3 |
0.1-0.3 |
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Temperature tolerance(℃) |
85 |
85 |
85 |
85 |
85 |
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* it is suggested that the chromatographic column with 5 μ m packing particle size, 5% cross-linking degree and 7.8 × 300 mm specification should be selected, and the flow rate range should be 0.1-0.2 ml / min.
The ion type of target carbohydrate series analytical column was selected according to the sample type
| Ion | Sample application |
| H | Fermentation products, fruit juices containing organic acids, alcohols and sugars |
| Ca | It is especially suitable for the separation of monosaccharide, disaccharide, trimeric sugar, tetrasaccharide and sugar alcohol |
| Pb |
Pentose and hexose in wood products Dairy products containing sucrose, lactose, etc |
| K | Sucrose, honey, corn syrup, beet sugar and other plant products containing sugars (containing zwitterionic ions such as betaine and trimethylammonium chloride) |
| Na | Low sugar, especially in the presence of heavy inorganic sodium ions, such as honey. |
High stability
Target carbohydrate series analytical chromatographic column adopts excellent filler manufacturing technology and advanced filling technology, which ensures the high stability of the packing. Even at high temperature, target carbohydrates can be stable in pure water and other water buffer systems. As shown in the figure below, the retention time of acid is only changed by 0.5% after continuous flushing 578 times of column volume at 55 ℃ for 6 days.
Column: 4.6x 250 mm
Mobile phase: 5mMH2SO4
Flow rate: 0.30 ml / min
Temperature: 55°C
Injection volume: 20μL
Detection: Ri
Sample:1, Citric acid (50 mM); 2, Aceticacid (100 mM)
Excellent inter assay reproducibility
The high inter batch reproducibility of target carbohydrate analytical column is due to the controllable surface chemical modification technology and strict monitoring of polymer resin production process. As shown in the figure below, the retention time error between different batches is within 0.25%.
Column: 7.8x300mm
Mobile phase: 2.5mMH2SO4
Flow rate:0.6ML/ min
Temperature: 55°C
Injection volume: 5μL
Detection: Ri
Sample:Mixture of Citric acid, Succunicacid, Formic acid, Acetic acid
Some application cases
Glucose fructose injection
Chromatographic : Target calcium 8% 9 μ m 7.8 * 300 mm
Mobile phase: ultrapure water
Flow rate: 0.6 ml / min
Temperature: 85 ° C
Detector: 1. Rid; 2.UV@200nim;3. UV@284NM
Injection volume: 20 μ L
Sample: glucose fructose injection
Peak:
1. Glucose
2. Fructose
3.5-hydroxymethylfurfural
Organic acids (maleic acid, malic acid and fumaric acid)
Chromatographic : Target hydrogen 8% 9um 7.8 * 300 mm
Mobile phase: 5mMH2SO4
Flow rate: 0.6 ML / min
Temperature: 40 °C
Detector: UV@210nm
Injection volume: 20μL
Sample (water as solvent matrix)
1.5μg / ml maleic acid
2.1mg/ml malic acid
10μg / ml fumaric acid
Mixing of organic acids and alcohols
chromatographic :Target-Hydrogen 8% 9um 7.8*300mm
Mobile phase: 2.5mMH2SO4
Flow rate: 0.6 mL/min
Temperature: 55%C
Detector: RID
Injection volume: 10 μL
Sample peak:
1. Maltotriose 2. Maltose
3. Glucose 4. Succinate
5. Lacticacid6. Glycerol
7. Acetic acid 8. Propylene glycol
9. Methanol10. Ethanol
11. Butanol
Ordering Information
| model | Ion type | Particle size | Specifications | Part No |
| Target-Calcium | Ca2+ | 5um | 7.8*300mm | 301811-78030 |
| Target-Calcium | Ca2+ | 8um | 7.8*300mm | 301818-78030 |
| Target-Calcium | Ca2+ | 6um | 7.8*300mm | 301816-78030 |
| Target-Calcium | Ca2+ | 9um | 7.8*300mm | 301819-78030 |
| Target-Calcium | Ca2+ | 10um | 7.8*300mm | 301809-78030 |
| Target-Hydrogen | H+ | 5um | 7.8*300mm | 301800-78030 |
| Target-Hydrogen | H+ | 8um | 7.8*300mm | 301828-78030 |
| Target-Hydrogen | H+ | 6um | 7.8*300mm | 301826-78030 |
| Target-Hydrogen | H+ | 9um | 7.8*300mm | 301812-78030 |
| Target-Hydrogen | H+ | 10um | 7.8*300mm | 301820-78030 |
| Target-Na | Na+ | 5um | 7.8*300mm | 301825-78030 |
| Target-Na | Na+ | 8um | 7.8*300mm | 301608-78030 |
| Target-Na | Na+ | 6um | 7.8*300mm | 301836-78030 |
| Target-Na | Na+ | 9um | 7.8*300mm | 301829-78030 |
| Target-Na | Na+ | 10um | 7.8*300mm | 301830-78030 |
| Target-Pb | Pb2+ | 5um | 7.8*300mm | 301700-78030 |
| Target-Pb | Pb2+ | 8um | 7.8*300mm | 301708-78030 |
| Target-Pb | Pb2+ | 6um | 7.8*300mm | 301846-78030 |
| Target-Pb | Pb2+ | 9um | 7.8*300mm | 301719-78030 |
| Target-Pb | Pb2+ | 10um | 7.8*300mm | 301710-78030 |
* tips: if you need more column with other crosslinking degree, particle size and specification, please consult your sales consultant.
